Problem with iqtree

I have this problem ??

Activating conda environment: /home/mamadou/Documents/ncov/.snakemake/conda/a3437ea545666bd29f3db7b9b3271b3e
WARNING: more threads requested than there are sequences; falling back to IQ-TREE’s -nt AUTO mode.

ERROR: Shell exited 2 when running: iqtree2 -ninit 2 -n 2 -me 0.05 -nt AUTO -s results/global/aligned-delim.fasta -m GTR -ninit 10 -n 4 > results/global/aligned-delim.iqtree.log
Command output was:
ERROR: Cannot read file results/global/aligned-delim.fasta

Building a tree via:
iqtree2 -ninit 2 -n 2 -me 0.05 -nt AUTO -s results/global/aligned-delim.fasta -m GTR -ninit 10 -n 4 > results/global/aligned-delim.iqtree.log
Nguyen et al: IQ-TREE: A fast and effective stochastic algorithm for estimating maximum likelihood phylogenies.
Mol. Biol. Evol., 32:268-274. https://doi.org/10.1093/molbev/msu300

ERROR: TREE BUILDING FAILED
Please see the log file for more details: results/global/aligned-delim.iqtree.log

Building original tree took 0.01925349235534668 seconds
Waiting at most 5 seconds for missing files.
MissingOutputException in line 665 of /home/mamadou/Documents/ncov/workflow/snakemake_rules/main_workflow.smk:
Job Missing files after 5 seconds:
results/global/tree_raw.nwk
This might be due to filesystem latency. If that is the case, consider to increase the wait time with --latency-wait.
Job id: 7 completed successfully, but some output files are missing. 7
Shutting down, this might take some time.
Exiting because a job execution failed. Look above for error message
Complete log: /home/mamadou/Documents/ncov/.snakemake/log/2021-09-14T184538.425957.snakemake.log

how i do ? thanks

Hi @Diaz13, welcome. Sorry you’re running into trouble running the build. In order to start troubleshooting this, we’d need to see the following files in /home/mamadou/Documents/ncov/:

  1. .snakemake/log/2021-09-14T184538.425957.snakemake.log
  2. results/global/aligned-delim.iqtree.log
  3. results/global/aligned-delim.fasta

Can you share those here by attaching them?