I am running augur to build phylogentic tree and want to visualize it on Auspice.us. Now there is a problem with my Diversity figure on Auspice.us that a part of gene, the “ORF1b”, is missing. Instead, there is a gene “ORF1ab” but in a wrong position, which is at 266…13468 but should stand at 266…21555). What should I do to modify the “ORF1ab” to right position or add “ORF1a” and “ORF1b” seperately? Any advices are valueable for me since I am a very green-hand on this.
Here are the screenshot and reference sequence used in Augur code. If any more info. needed please reply to me. Many thanks to every assistance!
Reference sequence download from NCBI named NC_045512_e.gb (Severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1, co - Nucleotide - NCBI)
(sry I can not upload it due to characters limitation)