Root is not the oldest

[Dana]

Hi ,

although I defined the root as oldest in augur refine, the root is not the oldest, and the dates are not accurate. How can I fix that?

attached is an image of the resulting tree.

Thank you,
Dana.

[rneher]

the root doesn’t necessarily correspond to the oldest sequence. But something went wrong in your analysis. You probably need to fix the clock rate and/or remove outlier sequences. Your root date is too early.

[Dana]

I removed an outlier sequence + added fixed clock rate and it worked.
Thank you!